Device

Part:BBa_K175045

Designed by: Tim_Weenink   Group: iGEM09_TUDelft   (2009-10-11)


Self Destructive Plasmid with GFP-LVA reporter

Final design of the Self Destructive Plasmid, consisting of a IPTG inducible I-SceI homing endonuclease, followed by its recognition site, followed by a anhydroTetracyclin (aTc) inducible GFP-LVA reporter gene (and a generator for TetR)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1167
    Illegal NheI site found at 1190
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2776


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