![](https://parts.igem.org/images/partbypart/icon_device.png)
Device
Part:BBa_K175045
Designed by: Tim_Weenink Group: iGEM09_TUDelft (2009-10-11)
Self Destructive Plasmid with GFP-LVA reporter
Final design of the Self Destructive Plasmid, consisting of a IPTG inducible I-SceI homing endonuclease, followed by its recognition site, followed by a anhydroTetracyclin (aTc) inducible GFP-LVA reporter gene (and a generator for TetR)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1167
Illegal NheI site found at 1190 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2776
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Categories
Parameters
None |